diff --git a/ProcessFASTQ.ipynb b/ProcessFASTQ.ipynb index ee2c1f13ee7f31fd7020bc6acd813e8b82e694f9..1ea5df5ea2e51ea2ee90ca8a3175b27f6441e70b 100644 --- a/ProcessFASTQ.ipynb +++ b/ProcessFASTQ.ipynb @@ -41,7 +41,7 @@ }, { "cell_type": "code", - "execution_count": 114, + "execution_count": 139, "metadata": { "collapsed": false }, @@ -95,7 +95,7 @@ }, { "cell_type": "code", - "execution_count": 115, + "execution_count": 140, "metadata": { "collapsed": false }, @@ -117,7 +117,7 @@ "flowcell362_lane4_pair1_Undetermined\n", "flowcell384_lane7_pair1_ACAGTG \t\t Processsed\n", "flowcell384_lane7_pair1_ACTTGA \t\t Processsed\n", - "flowcell384_lane7_pair1_CAGATC\n", + "flowcell384_lane7_pair1_CAGATC \t\t Processsed\n", "flowcell384_lane7_pair1_GATCAG\n", "flowcell384_lane7_pair1_TGACCA\n", "testing\n" @@ -149,8 +149,8 @@ "# fname = \"flowcell362_lane4_pair1_Undetermined\"\n", "# fname = \"flowcell384_lane7_pair1_ACAGTG\"\n", "# fname = \"flowcell384_lane7_pair1_ACTTGA\"\n", - "fname = \"flowcell384_lane7_pair1_CAGATC\"\n", - "# fname = \"flowcell384_lane7_pair1_GATCAG\"\n", + "# fname = \"flowcell384_lane7_pair1_CAGATC\"\n", + "fname = \"flowcell384_lane7_pair1_GATCAG\"\n", "# fname = \"flowcell384_lane7_pair1_TGACCA\"\n", "\n", "# Print available files in 0-Raws/ directory\n", @@ -208,7 +208,7 @@ }, { "cell_type": "code", - "execution_count": 116, + "execution_count": 141, "metadata": { "collapsed": false }, @@ -231,7 +231,7 @@ }, { "cell_type": "code", - "execution_count": 117, + "execution_count": 142, "metadata": { "collapsed": false }, @@ -239,10 +239,10 @@ { "data": { "text/plain": [ - "'flowcell384_lane7_pair1_CAGATC.fastq.gz'" + "'flowcell384_lane7_pair1_GATCAG.fastq.gz'" ] }, - "execution_count": 117, + "execution_count": 142, "metadata": {}, "output_type": "execute_result" } @@ -266,7 +266,7 @@ }, { "cell_type": "code", - "execution_count": 118, + "execution_count": 143, "metadata": { "collapsed": false }, @@ -276,12 +276,12 @@ "output_type": "stream", "text": [ "\n", - "flowcell384_lane7_pair1_CAGATC\n", + "flowcell384_lane7_pair1_GATCAG\n", "\n", - "@SN279:498:C88PKACXX:7:1109:1531:2224 1:N:0:CAGATC\n", - "NAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG\n", + "@SN279:498:C88PKACXX:7:1109:1982:2231 1:N:0:GATCAG\n", + "NATAGTGTAGTGGTTATCACTTTCGCTGTAGGCACCATCAATAGATCGGAA\n", "+\n", - "#1=DADEFHHHHHIIIIIHIIIIGIIIE?B??GB?FH;BCC3B)7888=DE\n" + "#1:DDFFFHFCFHCGIIJJJJJJAHHIJJIIIJJIJIJIIGIGIJJIJJII\n" ] } ], @@ -303,7 +303,7 @@ }, { "cell_type": "code", - "execution_count": 119, + "execution_count": 144, "metadata": { "collapsed": false }, @@ -312,7 +312,7 @@ "name": "stdout", "output_type": "stream", "text": [ - "Processing will take approximatively 0 hour(s) 38 minute(s) and 1 second(s)\n" + "Processing will take approximatively 0 hour(s) 30 minute(s) and 43 second(s)\n" ] } ], @@ -358,7 +358,7 @@ }, { "cell_type": "code", - "execution_count": 120, + "execution_count": null, "metadata": { "collapsed": false }, @@ -370,21 +370,11 @@ }, { "cell_type": "code", - "execution_count": 121, + "execution_count": null, "metadata": { "collapsed": false }, - "outputs": [ - { - "name": "stdout", - "output_type": "stream", - "text": [ - "This is cutadapt 1.9.1 with Python 3.5.1\n", - "Command line parameters: -a CTGTAGGCACCATCAATAGATCGGAA -o 1-Cutadapted/flowcell384_lane7_pair1_CAGATC.fastq.gz --quiet flowcell384_lane7_pair1_CAGATC.fastq.gz\n", - "Trimming 1 adapter with at most 10.0% errors in single-end mode ...\n" - ] - } - ], + "outputs": [], "source": [ "%%bash\n", "source ./source\n", @@ -402,19 +392,11 @@ }, { "cell_type": "code", - "execution_count": 122, + "execution_count": null, "metadata": { "collapsed": false }, - "outputs": [ - { - "name": "stdout", - "output_type": "stream", - "text": [ - "Cutadapt run time : 0:35:36.501319\n" - ] - } - ], + "outputs": [], "source": [ "# Store current time\n", "after = datetime.datetime.now()\n", @@ -427,7 +409,7 @@ }, { "cell_type": "code", - "execution_count": 123, + "execution_count": null, "metadata": { "collapsed": true }, @@ -448,7 +430,7 @@ }, { "cell_type": "code", - "execution_count": 124, + "execution_count": null, "metadata": { "collapsed": false }, @@ -460,7 +442,7 @@ }, { "cell_type": "code", - "execution_count": 125, + "execution_count": null, "metadata": { "collapsed": false }, @@ -475,19 +457,11 @@ }, { "cell_type": "code", - "execution_count": 126, + "execution_count": null, "metadata": { "collapsed": false }, - "outputs": [ - { - "name": "stdout", - "output_type": "stream", - "text": [ - "Zcat run time : 0:00:34.672678\n" - ] - } - ], + "outputs": [], "source": [ "# Store current time\n", "after = datetime.datetime.now()\n",